flag control (Addgene inc)
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flag control
Flag Control, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 115 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flag+ha+pcdna3+1/FLAG-HA-pcDNA3%2E1-+(Plasmid+%2352535)/pm41838122-98-5-7
Average 95 stars, based on 115 article reviews
Flag Control, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 115 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flag+ha+pcdna3+1/FLAG-HA-pcDNA3%2E1-+(Plasmid+%2352535)/pm41838122-98-5-7
Average 95 stars, based on 115 article reviews
flag control - by Bioz Stars,
2026-09
95/100 stars
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Plasmid Preparation:Article Title: A BRET-based Mpro Biosensor Containing a Nanobody and Tandem Cleavage Sites shows an Increased Cleavage Rate Article Snippet: .. Briefly, HEK 293T cells were co-transfected with one of the Mpro biosensors and pLVX-EF1alpha-SARS-CoV-2-nsp5–2× Strep-IRES-Puro (Addgene plasmid # 141,370) [41] or Article Title: NADPH oxidase subunit p22 phox : A marker of oxidase-dependent oxidative stress and target for stress suppression in nonphagocytic cells. Article Snippet: Reactive oxygen species (ROS)-producing NADPH oxidase (Nox) family proteins are involved in host defense.. The overproduction of ROS leads to oxidative stress, which is associated with a myriad of diseases.. The Nox subunit p22phox is essential for Nox1–4 activity, and p22phox and Nox2 proteins are mutually stabilized in phagocytic cells. Article Title: A BRET-based Mpro Biosensor Containing a Nanobody and Tandem Cleavage Sites shows an Increased Cleavage Rate Article Snippet: .. Briefly, HEK293T cells seeded onto 96-well white plates were transfected by combining the plasmid DNA (biosensor and Mpro), Opti-MEM (Invitrogen; 31,985,088) and 1.25 μg/well of PEI lipid (Sigma-Aldrich; 408,727–100 mL) and incubating them at room temperature for 30 min. Live cell Mpro biosensor proteolytic cleavage activity assays: Live cell Mpro proteolytic cleavage activity assays were performed by cotransfecting HEK293T cells with the respective Mpro BRET biosensor plasmid constructs along with either pLVX-EF1alpha-SARS-CoV-2nsp5–2× Strep-IRES-Puro (Mpro WT) (Addgene plasmid # 141,370) [41] or Article Title: FAM72A promotes UNG2 degradation and mutagenesis in human cancer cells Article Snippet: .. The coding sequence for human FAM72A was ordered from IDT and cloned into mammalian expression vector pcDNA3.1 and retroviral vector pMX-PIE using BamHI and NotI sites, and Article Title: dCas9-HDAC8-EGFP fusion enables epigenetic editing of breast cancer cells by H3K9 deacetylation. Article Snippet: Epigenetic editing is thriving as a robust tool for manipulating transcriptional regulation and cell fate.. Despite its regulatory role in gene downregulation, epigenetic editing with histone deacetylation has been sparsely studied, especially in the context of cancer.. In this current study, we have reconstructed a dCas9-HDAC8-EGFP fusion to perform histone deacetylation on the promoter of the ESR1, TERT and CDKN1C genes for the first time in breast cancer cell lines MCF-7 and MDA-MB-231 as well as in HEK293T cells. Article Title: FAM72A promotes UNG2 degradation and mutagenesis in human cancer cells. Article Snippet: .. The coding sequence for human FAM72A was ordered from IDT and cloned into mammalian expression vector pcDNA3.1 and retroviral vector pMX-PIE using BamHI and NotI sites, and Article Title: dCas9-HDAC8-EGFP fusion enables epigenetic editing of breast cancer cells by H3K9 deacetylation Article Snippet: .. Sonication:Article Title: A BRET-based Mpro Biosensor Containing a Nanobody and Tandem Cleavage Sites shows an Increased Cleavage Rate Article Snippet: .. Briefly, HEK 293T cells were co-transfected with one of the Mpro biosensors and pLVX-EF1alpha-SARS-CoV-2-nsp5–2× Strep-IRES-Puro (Addgene plasmid # 141,370) [41] or Transfection:Article Title: A BRET-based Mpro Biosensor Containing a Nanobody and Tandem Cleavage Sites shows an Increased Cleavage Rate Article Snippet: .. Briefly, HEK293T cells seeded onto 96-well white plates were transfected by combining the plasmid DNA (biosensor and Mpro), Opti-MEM (Invitrogen; 31,985,088) and 1.25 μg/well of PEI lipid (Sigma-Aldrich; 408,727–100 mL) and incubating them at room temperature for 30 min. Live cell Mpro biosensor proteolytic cleavage activity assays: Live cell Mpro proteolytic cleavage activity assays were performed by cotransfecting HEK293T cells with the respective Mpro BRET biosensor plasmid constructs along with either pLVX-EF1alpha-SARS-CoV-2nsp5–2× Strep-IRES-Puro (Mpro WT) (Addgene plasmid # 141,370) [41] or Activity Assay:Article Title: A BRET-based Mpro Biosensor Containing a Nanobody and Tandem Cleavage Sites shows an Increased Cleavage Rate Article Snippet: .. Briefly, HEK293T cells seeded onto 96-well white plates were transfected by combining the plasmid DNA (biosensor and Mpro), Opti-MEM (Invitrogen; 31,985,088) and 1.25 μg/well of PEI lipid (Sigma-Aldrich; 408,727–100 mL) and incubating them at room temperature for 30 min. Live cell Mpro biosensor proteolytic cleavage activity assays: Live cell Mpro proteolytic cleavage activity assays were performed by cotransfecting HEK293T cells with the respective Mpro BRET biosensor plasmid constructs along with either pLVX-EF1alpha-SARS-CoV-2nsp5–2× Strep-IRES-Puro (Mpro WT) (Addgene plasmid # 141,370) [41] or Bioluminescence Resonance Energy Transfer:Article Title: A BRET-based Mpro Biosensor Containing a Nanobody and Tandem Cleavage Sites shows an Increased Cleavage Rate Article Snippet: .. Briefly, HEK293T cells seeded onto 96-well white plates were transfected by combining the plasmid DNA (biosensor and Mpro), Opti-MEM (Invitrogen; 31,985,088) and 1.25 μg/well of PEI lipid (Sigma-Aldrich; 408,727–100 mL) and incubating them at room temperature for 30 min. Live cell Mpro biosensor proteolytic cleavage activity assays: Live cell Mpro proteolytic cleavage activity assays were performed by cotransfecting HEK293T cells with the respective Mpro BRET biosensor plasmid constructs along with either pLVX-EF1alpha-SARS-CoV-2nsp5–2× Strep-IRES-Puro (Mpro WT) (Addgene plasmid # 141,370) [41] or Construct:Article Title: A BRET-based Mpro Biosensor Containing a Nanobody and Tandem Cleavage Sites shows an Increased Cleavage Rate Article Snippet: .. Briefly, HEK293T cells seeded onto 96-well white plates were transfected by combining the plasmid DNA (biosensor and Mpro), Opti-MEM (Invitrogen; 31,985,088) and 1.25 μg/well of PEI lipid (Sigma-Aldrich; 408,727–100 mL) and incubating them at room temperature for 30 min. Live cell Mpro biosensor proteolytic cleavage activity assays: Live cell Mpro proteolytic cleavage activity assays were performed by cotransfecting HEK293T cells with the respective Mpro BRET biosensor plasmid constructs along with either pLVX-EF1alpha-SARS-CoV-2nsp5–2× Strep-IRES-Puro (Mpro WT) (Addgene plasmid # 141,370) [41] or Control:Article Title: A BRET-based Mpro Biosensor Containing a Nanobody and Tandem Cleavage Sites shows an Increased Cleavage Rate Article Snippet: .. Briefly, HEK293T cells seeded onto 96-well white plates were transfected by combining the plasmid DNA (biosensor and Mpro), Opti-MEM (Invitrogen; 31,985,088) and 1.25 μg/well of PEI lipid (Sigma-Aldrich; 408,727–100 mL) and incubating them at room temperature for 30 min. Live cell Mpro biosensor proteolytic cleavage activity assays: Live cell Mpro proteolytic cleavage activity assays were performed by cotransfecting HEK293T cells with the respective Mpro BRET biosensor plasmid constructs along with either pLVX-EF1alpha-SARS-CoV-2nsp5–2× Strep-IRES-Puro (Mpro WT) (Addgene plasmid # 141,370) [41] or Sequencing:Article Title: FAM72A promotes UNG2 degradation and mutagenesis in human cancer cells Article Snippet: .. The coding sequence for human FAM72A was ordered from IDT and cloned into mammalian expression vector pcDNA3.1 and retroviral vector pMX-PIE using BamHI and NotI sites, and Article Title: FAM72A promotes UNG2 degradation and mutagenesis in human cancer cells. Article Snippet: .. The coding sequence for human FAM72A was ordered from IDT and cloned into mammalian expression vector pcDNA3.1 and retroviral vector pMX-PIE using BamHI and NotI sites, and Clone Assay:Article Title: FAM72A promotes UNG2 degradation and mutagenesis in human cancer cells Article Snippet: .. The coding sequence for human FAM72A was ordered from IDT and cloned into mammalian expression vector pcDNA3.1 and retroviral vector pMX-PIE using BamHI and NotI sites, and Article Title: FAM72A promotes UNG2 degradation and mutagenesis in human cancer cells. Article Snippet: .. The coding sequence for human FAM72A was ordered from IDT and cloned into mammalian expression vector pcDNA3.1 and retroviral vector pMX-PIE using BamHI and NotI sites, and Article Title: The growth-promoting protein TMEM263 is an ER resident that controls early stages of lipid droplet biogenesis Article Snippet: mCherry-Sec61b (addgene #49155) was subcloned into pXLG3 (SpeI and KpnI). pLVXpuro-εCOPI-GFP was a gift from Prof David Stephens, University of Bristol (addgene #66604). .. TMEM263 cDNA (NP_001306590.1) was purchased from Integrated DNA Technologies (IDT) and cloned into pEGFP-C2 (Clontech), p-mCherry2-C1 (addgene #54563)), Expressing:Article Title: FAM72A promotes UNG2 degradation and mutagenesis in human cancer cells Article Snippet: .. The coding sequence for human FAM72A was ordered from IDT and cloned into mammalian expression vector pcDNA3.1 and retroviral vector pMX-PIE using BamHI and NotI sites, and Article Title: FAM72A promotes UNG2 degradation and mutagenesis in human cancer cells. Article Snippet: .. The coding sequence for human FAM72A was ordered from IDT and cloned into mammalian expression vector pcDNA3.1 and retroviral vector pMX-PIE using BamHI and NotI sites, and Retroviral:Article Title: FAM72A promotes UNG2 degradation and mutagenesis in human cancer cells Article Snippet: .. The coding sequence for human FAM72A was ordered from IDT and cloned into mammalian expression vector pcDNA3.1 and retroviral vector pMX-PIE using BamHI and NotI sites, and Article Title: FAM72A promotes UNG2 degradation and mutagenesis in human cancer cells. Article Snippet: .. The coding sequence for human FAM72A was ordered from IDT and cloned into mammalian expression vector pcDNA3.1 and retroviral vector pMX-PIE using BamHI and NotI sites, and |